Hotspot

rps16-trnQ-UUG

LSC · 6119-7542 · 1424 bp

Back to Ixora

Pi 0.0146
MarkerSeek score 57.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1424
variable_sites
31
pi
0.0146
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TCCACAATGGATCGTCTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 1519-1546 bp 1.0 80.2
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1497-1524 bp 1.0 80.2
rps16-trnQ-UUG_p3 GGATCGTCTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.8 58.1 1511-1538 bp 1.0 79.7
rps16-trnQ-UUG_p4 GATCGTCTCCTTCAAGTCGC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1510-1537 bp 1.0 77.7
rps16-trnQ-UUG_p5 CCACAATGGATCGTCTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.3 58.1 1518-1545 bp 1.0 75.7