Hotspot

ndhI

SSC · 118452-118952 · 501 bp

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Pi 0.507
MarkerSeek score 79.70
Primer available yes
Label ndhI

Feature Metadata

label_name
ndhI
feature_type
gene
region
SSC
length_bp
501
variable_sites
224
pi
0.507
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI_p1 CGCCGAGACCCCCTAATATA AGCCGAGTGTGAACGATTAC 55.0 50.0 58.2 58.0 1431 bp 0.5 58.7
ndhI_p2 CGCCGAGACCCCCTAATATA TTTGTGACCTTTGAGGAGCC 55.0 50.0 58.2 58.0 1637 bp 0.5 58.6
ndhI_p3 CGCCGAGACCCCCTAATATA GACCTTTGAGGAGCCGTATG 55.0 55.0 58.2 58.1 1632 bp 0.5 58.3
ndhI_p4 CGCCGAGACCCCCTAATATA GCAACAAGATGGACTTTGCC 55.0 50.0 58.2 57.9 1133 bp 0.5 58.2
ndhI_p5 TCCCAACGAAAAGGCAGAAT AGCCGAGTGTGAACGATTAC 45.0 50.0 57.7 58.0 1475 bp 0.5 57.6