Hotspot

ndhH

SSC · 121156-122337 · 1182 bp

Pi 0.471

MarkerSeek score 82.70

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 509
pi: 0.471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CGTTGTTGTATACCCGCAGA	CATATGCCCTGCCGTACATT	50.0	50.0	58.0	58.1	2024 bp	0.5	59.2
ndhH_p2	CCTGACATAAGAAGGCCGAC	CATATGCCCTGCCGTACATT	55.0	50.0	58.1	58.1	2288 bp	0.5	58.7
ndhH_p3	GACATAAGAAGGCCGACAGG	CATATGCCCTGCCGTACATT	55.0	50.0	58.1	58.1	2285 bp	0.5	58.7
ndhH_p4	CGTTGTTGTATACCCGCAGA	AGGTCTGCGGAAAATTCTGG	50.0	50.0	58.0	58.2	1576 bp	0.5	58.6
ndhH_p5	CGTTGTTGTATACCCGCAGA	GAAATGACGTCGGAAATGGC	50.0	50.0	58.0	57.8	1990 bp	0.5	58.6