Hotspot

ndhE

SSC · 117045-117350 · 306 bp

Pi 0.494

MarkerSeek score 73.00

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 119
pi: 0.494
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	ACATCCGTCCCAAGGTATCA	ATTTCCAGCTTTGGACCGTT	50.0	45.0	58.1	58.0	1128 bp	0.5	59.0
ndhE_p2	TGAGGATACCTTACGTGGCA	ATTTCCAGCTTTGGACCGTT	50.0	45.0	58.1	58.0	860 bp	0.5	58.8
ndhE_p3	AGGATACCTTACGTGGCAGA	ATTTCCAGCTTTGGACCGTT	50.0	45.0	57.8	58.0	858 bp	0.5	58.7
ndhE_p4	ACAACCGACACAATCCTCTG	ATTTCCAGCTTTGGACCGTT	50.0	45.0	57.8	58.0	1179 bp	0.5	58.6
ndhE_p5	CAACCGACACAATCCTCTGT	ATTTCCAGCTTTGGACCGTT	50.0	45.0	57.8	58.0	1178 bp	0.5	58.6