Hotspot

ndhA

SSC · 119031-121154 · 2124 bp

Pi 0.462

MarkerSeek score 80.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2124
variable_sites: 855
pi: 0.462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGACCAATATACCTTGCGGC	AATTGGGGTTTGTCAGGACC	50.0	50.0	58.0	58.0	2811 bp	0.5	59.6
ndhA_p2	TGACCAATATACCTTGCGGC	GTGGAGTCGCTGCTGATTTA	50.0	50.0	58.0	58.0	2975 bp	0.5	59.6
ndhA_p3	TGACCAATATACCTTGCGGC	TGGAGTCGCTGCTGATTTAC	50.0	50.0	58.0	58.0	2974 bp	0.5	59.6
ndhA_p4	TGACCAATATACCTTGCGGC	GCGCTATAAAATTCGCCCAC	50.0	50.0	58.0	58.0	2407 bp	0.5	59.4
ndhA_p5	TGACCAATATACCTTGCGGC	TCAACAGGCCCTAGAAGGAA	50.0	50.0	58.0	58.0	2632 bp	0.5	59.4