Hotspot

ccsA

SSC · 113717-114685 · 969 bp

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Pi 0.483
MarkerSeek score 76.40
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
969
variable_sites
324
pi
0.483
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.69
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 GTGAAATCGGTAGACACGCT GTTTTTGATTCTGGACCGCG 50.0 50.0 58.0 58.0 1557 bp 0.5 59.6
ccsA_p2 TGAAATCGGTAGACACGCTG GTTTTTGATTCTGGACCGCG 50.0 50.0 58.0 58.0 1556 bp 0.5 59.6
ccsA_p3 GTGAAATCGGTAGACACGCT GCTTGCATTACCGGGTATGA 50.0 50.0 58.0 58.0 1762 bp 0.5 59.6
ccsA_p4 TGAAATCGGTAGACACGCTG GCTTGCATTACCGGGTATGA 50.0 50.0 58.0 58.0 1761 bp 0.5 59.6
ccsA_p5 TCCCCATCGACCTTTACCTT GTTTTTGATTCTGGACCGCG 50.0 50.0 58.0 58.0 2114 bp 0.5 59.4