Hotspot

rps15-ycf1

SSC · 122707-123103 · 397 bp

Back to Ixeris

Pi 0.487
MarkerSeek score 76.70
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
397
variable_sites
165
pi
0.487
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 CCGCAGACCTCTCTGAGATA AATAATGGGAGCCGCTTGAG 55.0 50.0 58.0 58.0 613 bp 0.5 59.6
rps15-ycf1_p2 CCGCAGACCTCTCTGAGATA GAATAATGGGAGCCGCTTGA 55.0 50.0 58.0 58.0 614 bp 0.5 59.5
rps15-ycf1_p3 AACACCATGCATTGATGGGT AATAATGGGAGCCGCTTGAG 45.0 50.0 58.1 58.0 889 bp 0.5 59.3
rps15-ycf1_p4 AACACCATGCATTGATGGGT GAATAATGGGAGCCGCTTGA 45.0 50.0 58.1 58.0 890 bp 0.5 59.3
rps15-ycf1_p5 CCGCAGACCTCTCTGAGATA TGCCCGCCTATCTCTAAGAA 55.0 50.0 58.0 57.9 1031 bp 0.5 59.1