Hotspot

ndhG-ndhI

SSC · 118100-118451 · 352 bp

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Pi 0.423
MarkerSeek score 75.70
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
352
variable_sites
126
pi
0.423
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.85
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 CGCCGAGACCCCCTAATATA GCAACAAGATGGACTTTGCC 55.0 50.0 58.2 57.9 1133 bp 0.5 58.2
ndhG-ndhI_p2 CGCCGAGACCCCCTAATATA TTGATCACATCCGAGCGTTT 55.0 45.0 58.2 57.9 791 bp 0.5 58.0
ndhG-ndhI_p3 TGAATCCCGATTTGTTGGCT GCAACAAGATGGACTTTGCC 45.0 50.0 57.8 57.9 1497 bp 0.5 57.7
ndhG-ndhI_p4 TGAATCCCGATTTGTTGGCT TTGATCACATCCGAGCGTTT 45.0 45.0 57.8 57.9 1155 bp 0.5 57.5
ndhG-ndhI_p5 CGCCGAGACCCCCTAATATA ACAAGATGGACTTTGCCGAG 55.0 50.0 58.2 58.2 1130 bp 0.5 57.3