Hotspot

ndhD-psaC

SSC · 116448-116562 · 115 bp

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Pi 0.485
MarkerSeek score 75.80
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
115
variable_sites
50
pi
0.485
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 TCTTTCCTCCCCACATGGAT ATGGTTAGGGCGCTTATGTG 50.0 50.0 58.0 58.0 1300 bp 0.5 59.6
ndhD-psaC_p2 TCTTTCCTCCCCACATGGAT CTGCTGAAGCTGCTATTGGA 50.0 50.0 58.0 58.0 1167 bp 0.5 59.6
ndhD-psaC_p3 TCTTTCCTCCCCACATGGAT GCTGAAGCTGCTATTGGACT 50.0 50.0 58.0 58.0 1165 bp 0.5 59.6
ndhD-psaC_p4 GAGACGTTTCTTTCCTCCCC ATGGTTAGGGCGCTTATGTG 55.0 50.0 57.9 58.0 1308 bp 0.5 59.0
ndhD-psaC_p5 GAGACGTTTCTTTCCTCCCC CTGCTGAAGCTGCTATTGGA 55.0 50.0 57.9 58.0 1175 bp 0.5 59.0