Hotspot

rpl32-trnL-UAG

SSC · 112748-113522 · 775 bp

Back to Ixeris

Pi 0.448
MarkerSeek score 78.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
775
variable_sites
263
pi
0.448
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.76
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAAGGAAAGGGTATTGGGCG AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 908 bp 0.5 58.7
rpl32-trnL-UAG_p2 AAAGGAAAGGGTATTGGGCG CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 909 bp 0.5 58.7
rpl32-trnL-UAG_p3 GTATTGGGCGGCGTTAAAAG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.8 58.0 898 bp 0.5 58.5
rpl32-trnL-UAG_p4 GTATTGGGCGGCGTTAAAAG CAGCGTGTCTACCGATTTCA 50.0 50.0 57.8 58.0 899 bp 0.5 58.5
rpl32-trnL-UAG_p5 AAAGGAAAGGGTATTGGGCG GAAGTAGCAAAGCCTTGGGT 50.0 50.0 58.2 58.1 1417 bp 0.5 58.1