Hotspot

ndhF-rpl32

SSC · 111495-112582 · 1088 bp

Back to Ixeris

Pi 0.428
MarkerSeek score 81.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1088
variable_sites
400
pi
0.428
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.86
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGTCGGAAAAAGAAG AAGGTAAAGGTCGATGGGGA 50.0 50.0 58.0 58.0 1598 bp 0.5 59.4
ndhF-rpl32_p2 GTTGCCGTCGGAAAAAGAAG AGGTAAAGGTCGATGGGGAA 50.0 50.0 58.0 58.0 1597 bp 0.5 59.4
ndhF-rpl32_p3 TGCCGTCGGAAAAAGAAGAA AAGGTAAAGGTCGATGGGGA 45.0 50.0 58.0 58.0 1596 bp 0.5 59.3
ndhF-rpl32_p4 TGCCGTCGGAAAAAGAAGAA AGGTAAAGGTCGATGGGGAA 45.0 50.0 58.0 58.0 1595 bp 0.5 59.3
ndhF-rpl32_p5 TTGCCGTCGGAAAAAGAAGA AGGTAAAGGTCGATGGGGAA 45.0 50.0 58.0 58.0 1596 bp 0.5 59.3