Hotspot

trnH-GUG-psbA

LSC · 79-490 · 412 bp

Back to Ixeris

Pi 0.0537
MarkerSeek score 83.60
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
412
variable_sites
21
pi
0.0537
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCGTGCTAACCTTGGTATGG 55.0 55.0 58.1 58.3 512-530 bp 1.0 82.7
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 526-544 bp 1.0 82.5
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 525-543 bp 1.0 82.5
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 527-545 bp 1.0 82.5
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG ACCGTGCTAACCTTGGTATG 55.0 50.0 58.1 57.6 513-531 bp 1.0 82.2