Hotspot

ndhF

SSC · 111359-113611 · 2253 bp

Back to Ivesia

Pi 0.000888
MarkerSeek score 44.30
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2253
variable_sites
2
pi
0.000888
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 AGAAGATCCTTCCCCTTCCC TGAGTCAAGTTATGGGCCCT 55.0 50.0 58.1 58.3 2969-2971 bp 1.0 77.3
ndhF_p2 GAAGATCCTTCCCCTTCCCT TGAGTCAAGTTATGGGCCCT 55.0 50.0 58.1 58.3 2968-2970 bp 1.0 77.3
ndhF_p3 GCAGAAGATCCTTCCCCTTC TGAGTCAAGTTATGGGCCCT 55.0 50.0 57.7 58.3 2971-2973 bp 1.0 75.3
ndhF_p4 AAGATCCTTCCCCTTCCCTT TGAGTCAAGTTATGGGCCCT 50.0 50.0 57.6 58.3 2967-2969 bp 1.0 75.0
ndhF_p5 AGATCCTTCCCCTTCCCTTT TGAGTCAAGTTATGGGCCCT 50.0 50.0 57.6 58.3 2966-2968 bp 1.0 75.0