Hotspot

ycf1-ndhF

SSC · 111305-111358 · 54 bp

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Pi 0.125
MarkerSeek score 90.60
Primer available yes
Label ycf1-ndhF

Feature Metadata

label_name
ycf1-ndhF
feature_type
igs
region
SSC
length_bp
54
variable_sites
6
pi
0.125
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
90.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-ndhF_p1 TCTGGATGGGAATCAAGAACA GGGCGGTCGAATTTCTTCTT 42.9 50.0 56.6 58.5 221-230 bp 1.0 62.7
ycf1-ndhF_p2 TCTGGATGGGAATCAAGAACAA GGGCGGTCGAATTTCTTCTT 40.9 50.0 57.3 58.5 221-230 bp 1.0 60.5
ycf1-ndhF_p3 TGGATGGGAATCAAGAACAAGA GGGCGGTCGAATTTCTTCTT 40.9 50.0 57.3 58.5 219-228 bp 1.0 60.5
ycf1-ndhF_p4 ATCTGGATGGGAATCAAGAACA GGGCGGTCGAATTTCTTCTT 40.9 50.0 57.1 58.5 222-231 bp 1.0 58.8
ycf1-ndhF_p5 TCTGGATGGGAATCAAGAACA GGTATCAAATCTGTAGGGGGC 42.9 52.4 56.6 57.9 238-247 bp 1.0 57.8