Hotspot

trnP-UGG-psaJ

LSC · 67250-67673 · 424 bp

Back to Ivesia

Pi 0.00472
MarkerSeek score 57.80
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
424
variable_sites
2
pi
0.00472
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 TCGAACCCGTGACATTTTGT GGAATATCAACGCATCCGGA 45.0 50.0 58.0 57.8 601-649 bp 1.0 78.6
trnP-UGG-psaJ_p2 GGATGACAGGATTCGAACCC GGAATATCAACGCATCCGGA 55.0 50.0 58.0 57.8 613-661 bp 1.0 78.6
trnP-UGG-psaJ_p3 GGGATGACAGGATTCGAACC GGAATATCAACGCATCCGGA 55.0 50.0 58.0 57.8 614-662 bp 1.0 78.6
trnP-UGG-psaJ_p4 TCGAACCCGTGACATTTTGT AGTACTTACTACCGGTGCCA 45.0 50.0 58.0 57.8 531-579 bp 1.0 78.2
trnP-UGG-psaJ_p5 ACCCGTGACATTTTGTACCC GGAATATCAACGCATCCGGA 50.0 50.0 58.1 57.8 597-645 bp 1.0 78.1