Hotspot

rbcL-accD

LSC · 57392-58049 · 658 bp

Back to Ivesia

Pi 0.00462
MarkerSeek score 62.70
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
658
variable_sites
3
pi
0.00462
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GAGTCCTGAACTAGCTGCTG CTTAGCCTACACCCGTGTTC 55.0 55.0 57.7 58.0 790-799 bp 1.0 78.8
rbcL-accD_p2 GAGTCCTGAACTAGCTGCTG TACTTAGCCTACACCCGTGT 55.0 50.0 57.7 57.8 792-801 bp 1.0 77.1
rbcL-accD_p3 GAGTCCTGAACTAGCTGCTG GCGAACCAAACCACCATTTT 55.0 45.0 57.7 57.8 750-759 bp 1.0 77.1
rbcL-accD_p4 GAGTCCTGAACTAGCTGCTG AACAAGAGCGAACCAAACCA 55.0 45.0 57.7 58.2 757-766 bp 1.0 77.0
rbcL-accD_p5 GCTGCTGCTTGTGAAGTATG CTTAGCCTACACCCGTGTTC 50.0 55.0 57.5 58.0 777-786 bp 1.0 76.9