Hotspot

rps16-trnQ-UUG

LSC · 6290-7247 · 958 bp

Back to Ivesia

Pi 0.00626
MarkerSeek score 55.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
958
variable_sites
6
pi
0.00626
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCGGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1049-1101 bp 1.0 79.6
rps16-trnQ-UUG_p2 GCGGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1047-1099 bp 1.0 79.6
rps16-trnQ-UUG_p3 GATGTAAGACTCCACAGCGG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 1063-1115 bp 1.0 79.3
rps16-trnQ-UUG_p4 GGATGTAAGACTCCACAGCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 1064-1116 bp 1.0 79.3
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1031-1083 bp 1.0 78.7