Hotspot

trnK-UUU-rps16

LSC · 4318-5137 · 820 bp

Back to Ivesia

Pi 0.00366
MarkerSeek score 51.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
820
variable_sites
3
pi
0.00366
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT ACGGAACTTCGCGTTAATCA 50.0 45.0 58.2 57.9 893 bp 1.0 78.4
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT ACGGAACTTCGCGTTAATCA 50.0 45.0 58.2 57.9 892 bp 1.0 78.4
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA ACGGAACTTCGCGTTAATCA 50.0 45.0 58.3 57.9 902 bp 1.0 77.5
trnK-UUU-rps16_p4 TACCGTTGAGTTAGCAACCC ACGGAACTTCGCGTTAATCA 50.0 45.0 57.5 57.9 879 bp 1.0 76.0
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GCGGGAGTTTTTACGGAACT 50.0 50.0 58.2 58.5 904 bp 1.0 75.0