Hotspot

matK-trnK-UUU

LSC · 3540-4280 · 741 bp

Back to Ivesia

Pi 0.00272
MarkerSeek score 61.10
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
741
variable_sites
2
pi
0.00272
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 CGATCATGAGCAAGGGCATA AACGGTAGAGTACTCGGCTT 50.0 50.0 57.8 58.2 863-869 bp 1.0 78.0
matK-trnK-UUU_p2 CGATCATGAGCAAGGGCATA ACGGTAGAGTACTCGGCTTT 50.0 50.0 57.8 58.2 862-868 bp 1.0 78.0
matK-trnK-UUU_p3 AACCACGATCATGAGCAAGG AACGGTAGAGTACTCGGCTT 50.0 50.0 58.3 58.2 868-874 bp 1.0 77.3
matK-trnK-UUU_p4 AACCACGATCATGAGCAAGG ACGGTAGAGTACTCGGCTTT 50.0 50.0 58.3 58.2 867-873 bp 1.0 77.3
matK-trnK-UUU_p5 CGATCATGAGCAAGGGCATA TTGGGTTGCTAACTCAACGG 50.0 50.0 57.8 58.4 878-884 bp 1.0 76.1