Hotspot

ycf1

SSC · 129221-134845 · 5625 bp

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Pi 0.339
MarkerSeek score 73.10
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5625
variable_sites
1522
pi
0.339
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.80
markerseek_score
73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCTGTGCCAAGGTTTCAGAC TTCTTGAACGGGCCTTATCG 50.0 50.0 58.0 58.0 1191 bp 0.5 59.6
ycf1_p2 TCTGTGCCAAGGTTTCAGAC GGGCCTTATCGTGGAACAAT 50.0 50.0 58.0 57.9 1182 bp 0.5 59.3
ycf1_p3 AGCTTCCATTCCCCAAACTG TTCTTGAACGGGCCTTATCG 50.0 50.0 58.1 58.0 1287 bp 0.5 59.3
ycf1_p4 CAGCTTCCATTCCCCAAACT TTCTTGAACGGGCCTTATCG 50.0 50.0 58.1 58.0 1288 bp 0.5 59.3
ycf1_p5 TCAGCTTCCATTCCCCAAAC TTCTTGAACGGGCCTTATCG 50.0 50.0 58.1 58.0 1289 bp 0.5 59.2