Hotspot

rpl32

SSC · 118506-118664 · 159 bp

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Pi 0.377
MarkerSeek score 75.20
Primer available yes
Label rpl32

Feature Metadata

label_name
rpl32
feature_type
gene
region
SSC
length_bp
159
variable_sites
52
pi
0.377
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32_p1 GCTCACCTGCAACCAATCTT GAATCCCCACCCCTGAAATG 50.0 55.0 58.7 58.2 824 bp 0.5 52.3
rpl32_p2 GCTCACCTGCAACCAATCTT TCCCCACCCCTGAAATGATA 50.0 50.0 58.7 57.7 821 bp 0.5 51.6
rpl32_p3 GCTCACCTGCAACCAATCTT AGAATCCCCACCCCTGAAAT 50.0 50.0 58.7 58.3 825 bp 0.5 51.6
rpl32_p4 GCTCACCTGCAACCAATCTT TAAGAATCCCCACCCCTGAA 50.0 50.0 58.7 57.6 827 bp 0.5 51.1
rpl32_p5 GCTCACCTGCAACCAATCTT ATCCCCACCCCTGAAATGAT 50.0 50.0 58.7 58.4 822 bp 0.5 51.0