Hotspot

ndhI

SSC · 124478-124978 · 501 bp

Pi 0.443

MarkerSeek score 76.60

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 501
variable_sites: 171
pi: 0.443
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	GTGCAGCAGCTACAAAATGG	AAAGCAGGTGGAGTCTTTGG	50.0	50.0	58.0	58.0	1352 bp	0.5	59.8
ndhI_p2	GTGCAGCAGCTACAAAATGG	TACTTGGGTGGGTGGAATCT	50.0	50.0	58.0	57.9	1415 bp	0.5	59.5
ndhI_p3	GTGCAGCAGCTACAAAATGG	TCTTCCCAACTCCTGTCACT	50.0	50.0	58.0	57.9	1169 bp	0.5	59.1
ndhI_p4	ACAAACCGTCGAAGTAACCC	AAAGCAGGTGGAGTCTTTGG	50.0	50.0	58.1	58.0	1480 bp	0.5	58.9
ndhI_p5	GAAGTAACCCCATCCCCAAC	AAAGCAGGTGGAGTCTTTGG	55.0	50.0	57.9	58.0	1470 bp	0.5	58.8