Hotspot

ndhH

SSC · 127263-128444 · 1182 bp

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Pi 0.492
MarkerSeek score 82.00
Primer available yes
Label ndhH

Feature Metadata

label_name
ndhH
feature_type
gene
region
SSC
length_bp
1182
variable_sites
519
pi
0.492
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH_p1 TACGTTGTTGTATCCCTGCG AAAGAAGAAAGCAGGGGGTC 50.0 50.0 58.0 57.7 1705 bp 0.5 57.6
ndhH_p2 TACGTTGTTGTATCCCTGCG TTCTGGGAAAACGTCAACGA 50.0 45.0 58.0 57.7 1581 bp 0.5 57.5
ndhH_p3 TACGTTGTTGTATCCCTGCG TTGGTCGGTTGGATATTCGG 50.0 50.0 58.0 57.7 1512 bp 0.5 57.4
ndhH_p4 TTGGAGAATTCCCAAAGGGC AAAGAAGAAAGCAGGGGGTC 50.0 50.0 58.1 57.7 1743 bp 0.5 57.1
ndhH_p5 TACGTTGTTGTATCCCTGCG GCAGGGGGTCTGTTGAATTT 50.0 50.0 58.0 58.4 1695 bp 0.5 57.1