Hotspot

ndhF

SSC · 115388-117601 · 2214 bp

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Pi 0.464
MarkerSeek score 81.30
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2214
variable_sites
929
pi
0.464
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 GCAGAATACCGTCACCCATT AGTATTTTGGAAAGACGCCGA 50.0 42.9 58.0 58.0 2917 bp 0.5 51.3
ndhF_p2 AAGAGGGATCCACCGAAGAA AGTATTTTGGAAAGACGCCGA 50.0 42.9 58.0 58.0 2785 bp 0.5 51.2
ndhF_p3 AGAGGGATCCACCGAAGAAT AGTATTTTGGAAAGACGCCGA 50.0 42.9 57.8 58.0 2784 bp 0.5 50.2
ndhF_p4 TCCGAAACGAAGGGGACTAA AGTATTTTGGAAAGACGCCGA 50.0 42.9 58.4 58.0 2813 bp 0.5 48.7
ndhF_p5 TTCCGAAACGAAGGGGACTA AGTATTTTGGAAAGACGCCGA 50.0 42.9 58.4 58.0 2814 bp 0.5 48.7