Hotspot

ndhD

SSC · 120860-122362 · 1503 bp

Pi 0.481

MarkerSeek score 80.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 613
pi: 0.481
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AGGTGCAAATTCCGCAATTG	ATCTCTCATGAGCCGATCCA	45.0	50.0	57.9	58.0	2318 bp	0.5	59.3
ndhD_p2	CAAGGTGCAAATTCCGCAAT	ATCTCTCATGAGCCGATCCA	45.0	50.0	57.9	58.0	2320 bp	0.5	59.3
ndhD_p3	GAGGCATGGGGGTCATATTG	ATCTCTCATGAGCCGATCCA	55.0	50.0	58.1	58.0	2425 bp	0.5	59.3
ndhD_p4	AGGTGCAAATTCCGCAATTG	CGTTTCAGAAAACTCCACGC	45.0	50.0	57.9	58.0	1918 bp	0.5	59.2
ndhD_p5	AGGTGCAAATTCCGCAATTG	GTTTCAGAAAACTCCACGCG	45.0	50.0	57.9	58.0	1917 bp	0.5	59.2