Hotspot

rps15-ycf1

SSC · 128825-129220 · 396 bp

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Pi 0.451
MarkerSeek score 77.20
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
396
variable_sites
156
pi
0.451
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TTCCTAACTGCTCTGGTCCA GCCGGTTCAGTATGGTAAGG 50.0 55.0 58.0 58.1 1097 bp 0.5 59.4
rps15-ycf1_p2 AACACCATGCATTGATGGGT GCCGGTTCAGTATGGTAAGG 45.0 55.0 58.1 58.1 901 bp 0.5 59.1
rps15-ycf1_p3 TGCATTGATGGGTGATGAGG GCCGGTTCAGTATGGTAAGG 50.0 55.0 57.9 58.1 894 bp 0.5 59.1
rps15-ycf1_p4 TTCCTAACTGCTCTGGTCCA AAAGGGGCGAAATGAGTCTG 50.0 50.0 58.0 58.2 1655 bp 0.5 58.4
rps15-ycf1_p5 TTCCTAACTGCTCTGGTCCA CAAAGGGGCGAAATGAGTCT 50.0 50.0 58.0 58.2 1656 bp 0.5 58.4