Hotspot

ndhH-rps15

SSC · 128445-128551 · 107 bp

Back to Itea

Pi 0.41
MarkerSeek score 76.90
Primer available yes
Label ndhH-rps15

Feature Metadata

label_name
ndhH-rps15
feature_type
igs
region
SSC
length_bp
107
variable_sites
41
pi
0.41
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-rps15_p1 ACTCCCTCAACCCGTTCTAA GCCGGTTCAGTATGGTAAGG 50.0 55.0 58.0 58.1 1443 bp 0.5 59.4
ndhH-rps15_p2 TTCCTAACTGCTCTGGTCCA GCCGGTTCAGTATGGTAAGG 50.0 55.0 58.0 58.1 1097 bp 0.5 59.4
ndhH-rps15_p3 TTCCTCTCCGCCAATAATGC GCCGGTTCAGTATGGTAAGG 50.0 55.0 58.0 58.1 1465 bp 0.5 59.3
ndhH-rps15_p4 TTTATTGCTTCCTCTCCGCC GCCGGTTCAGTATGGTAAGG 50.0 55.0 58.0 58.1 1473 bp 0.5 59.3
ndhH-rps15_p5 TCTGTGCACCAATATCTGCC GCCGGTTCAGTATGGTAAGG 50.0 55.0 58.0 58.1 1211 bp 0.5 59.3