Hotspot

trnL-UAG-ccsA

SSC · 119623-119711 · 89 bp

Back to Itea

Pi 0.412
MarkerSeek score 76.90
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
89
variable_sites
35
pi
0.412
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 GTGAAATCGGTAGACACGCT AGCAAAGCCCTGGGTAAAAA 50.0 45.0 58.0 57.9 499 bp 0.5 59.2
trnL-UAG-ccsA_p2 TGAAATCGGTAGACACGCTG AGCAAAGCCCTGGGTAAAAA 50.0 45.0 58.0 57.9 498 bp 0.5 59.2
trnL-UAG-ccsA_p3 GTGAAATCGGTAGACACGCT CATTAGCCCATACTGCTCCC 50.0 55.0 58.0 57.8 902 bp 0.5 58.4
trnL-UAG-ccsA_p4 TGAAATCGGTAGACACGCTG CATTAGCCCATACTGCTCCC 50.0 55.0 58.0 57.8 901 bp 0.5 58.4
trnL-UAG-ccsA_p5 GTGAAATCGGTAGACACGCT GTAGCAAAGCCCTGGGTAAA 50.0 50.0 58.0 57.8 501 bp 0.5 58.3