Hotspot

rpl32-trnL-UAG

SSC · 118665-119542 · 878 bp

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Pi 0.446
MarkerSeek score 81.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
878
variable_sites
344
pi
0.446
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GAAAGGGATATTGGGCAGCA AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 1002 bp 0.5 59.4
rpl32-trnL-UAG_p2 GAAAGGGATATTGGGCAGCA CAGCGTGTCTACCGATTTCA 50.0 50.0 57.9 58.0 1003 bp 0.5 59.4
rpl32-trnL-UAG_p3 GAAAGGGATATTGGGCAGCA TTAGAAGATTCCATGCCGCC 50.0 50.0 57.9 58.0 1066 bp 0.5 59.2
rpl32-trnL-UAG_p4 GAAAGGGATATTGGGCAGCA AGCAAAGCCCTGGGTAAAAA 50.0 45.0 57.9 57.9 1481 bp 0.5 58.7
rpl32-trnL-UAG_p5 GAAAGGGATATTGGGCAGCA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.9 57.9 1012 bp 0.5 58.4