primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	psbC-trnS-UGA	1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	20	20	55.0	55.0	58.2	58.0	0.0	0.0	0.181932	38618	38839	343-345	1.000000	80.9
psbC-trnS-UGA_p2	psbC-trnS-UGA	2	CCTGTTCTTTCCATGACCCC	GTCTTTCGGAGAGATGGCTG	20	20	55.0	55.0	58.2	58.1	0.0	1.2	0.227330	38618	38839	351-353	1.000000	80.5
psbC-trnS-UGA_p3	psbC-trnS-UGA	3	TCGTAGGTCATTTGTGGCAC	GAGAGATGGCTGAGTGGTTG	20	20	50.0	55.0	58.2	58.0	11.6	0.0	0.218021	38618	38839	423-425	1.000000	80.1
psbC-trnS-UGA_p4	psbC-trnS-UGA	4	CCTGTTCTTTCCATGACCCC	TCTTTCGGAGAGATGGCTGA	20	20	55.0	50.0	58.2	58.1	0.0	0.0	0.307944	38618	38839	350-352	1.000000	79.8
psbC-trnS-UGA_p5	psbC-trnS-UGA	5	TCGTAGGTCATTTGTGGCAC	GTCTTTCGGAGAGATGGCTG	20	20	50.0	55.0	58.2	58.1	11.6	1.2	0.263419	38618	38839	431-433	1.000000	79.7
ndhF_p1	ndhF	1	GCAGAATACCGTCACCCATT	AGTATTTTGGAAAGACGCCGA	20	21	50.0	42.9	58.0	58.0	0.0	0.0	1.086724	115388	117601	2917-2917	0.500000	51.3
ndhF_p2	ndhF	2	AAGAGGGATCCACCGAAGAA	AGTATTTTGGAAAGACGCCGA	20	21	50.0	42.9	58.0	58.0	15.2	0.0	1.095716	115388	117601	2785-2785	0.500000	51.2
ndhF_p3	ndhF	3	AGAGGGATCCACCGAAGAAT	AGTATTTTGGAAAGACGCCGA	20	21	50.0	42.9	57.8	58.0	15.2	0.0	1.230853	115388	117601	2784-2784	0.500000	50.2
ndhF_p4	ndhF	4	TCCGAAACGAAGGGGACTAA	AGTATTTTGGAAAGACGCCGA	20	21	50.0	42.9	58.4	58.0	0.0	0.0	1.416932	115388	117601	2813-2813	0.500000	48.7
ndhF_p5	ndhF	5	TTCCGAAACGAAGGGGACTA	AGTATTTTGGAAAGACGCCGA	20	21	50.0	42.9	58.4	58.0	0.0	0.0	1.416932	115388	117601	2814-2814	0.500000	48.7
ndhF-rpl32_p1	ndhF-rpl32	1	TGTTACCGCCGGAAAAAGAA	TGCTGCCCAATATCCCTTTC	20	20	45.0	50.0	58.0	57.9	11.7	0.0	0.107225	117602	118505	1087-1087	0.500000	59.1
ndhF-rpl32_p2	ndhF-rpl32	2	TTGTTACCGCCGGAAAAAGA	TGCTGCCCAATATCCCTTTC	20	20	45.0	50.0	58.0	57.9	11.7	0.0	0.107225	117602	118505	1088-1088	0.500000	59.1
ndhF-rpl32_p3	ndhF-rpl32	3	GCCCACATACGACGAAGATT	TGCTGCCCAATATCCCTTTC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.135845	117602	118505	1110-1110	0.500000	58.9
ndhF-rpl32_p4	ndhF-rpl32	4	ACGAATGGGATGATCCATGC	TGCTGCCCAATATCCCTTTC	20	20	50.0	50.0	58.1	57.9	14.4	0.0	0.170187	117602	118505	1029-1029	0.500000	58.6
ndhF-rpl32_p5	ndhF-rpl32	5	CGAATGGGATGATCCATGCA	TGCTGCCCAATATCCCTTTC	20	20	50.0	50.0	58.1	57.9	14.4	0.0	0.171532	117602	118505	1028-1028	0.500000	58.6
rpl32_p1	rpl32	1	GCTCACCTGCAACCAATCTT	GAATCCCCACCCCTGAAATG	20	20	50.0	55.0	58.7	58.2	0.0	0.0	0.968247	118506	118664	824-824	0.500000	52.3
rpl32_p2	rpl32	2	GCTCACCTGCAACCAATCTT	TCCCCACCCCTGAAATGATA	20	20	50.0	50.0	58.7	57.7	0.0	0.0	1.044018	118506	118664	821-821	0.500000	51.6
rpl32_p3	rpl32	3	GCTCACCTGCAACCAATCTT	AGAATCCCCACCCCTGAAAT	20	20	50.0	50.0	58.7	58.3	0.0	0.0	1.049875	118506	118664	825-825	0.500000	51.6
rpl32_p4	rpl32	4	GCTCACCTGCAACCAATCTT	TAAGAATCCCCACCCCTGAA	20	20	50.0	50.0	58.7	57.6	0.0	0.0	1.118417	118506	118664	827-827	0.500000	51.1
rpl32_p5	rpl32	5	GCTCACCTGCAACCAATCTT	ATCCCCACCCCTGAAATGAT	20	20	50.0	50.0	58.7	58.4	0.0	0.0	1.124576	118506	118664	822-822	0.500000	51.0
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GAAAGGGATATTGGGCAGCA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.079608	118665	119542	1002-1002	0.500000	59.4
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GAAAGGGATATTGGGCAGCA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.080978	118665	119542	1003-1003	0.500000	59.4
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GAAAGGGATATTGGGCAGCA	TTAGAAGATTCCATGCCGCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.104459	118665	119542	1066-1066	0.500000	59.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GAAAGGGATATTGGGCAGCA	AGCAAAGCCCTGGGTAAAAA	20	20	50.0	45.0	57.9	57.9	0.0	15.6	0.167382	118665	119542	1481-1481	0.500000	58.7
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GAAAGGGATATTGGGCAGCA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.196055	118665	119542	1012-1012	0.500000	58.4
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	GTGAAATCGGTAGACACGCT	AGCAAAGCCCTGGGTAAAAA	20	20	50.0	45.0	58.0	57.9	0.0	15.6	0.103434	119623	119711	499-499	0.500000	59.2
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	TGAAATCGGTAGACACGCTG	AGCAAAGCCCTGGGTAAAAA	20	20	50.0	45.0	58.0	57.9	0.0	15.6	0.104805	119623	119711	498-498	0.500000	59.2
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	GTGAAATCGGTAGACACGCT	CATTAGCCCATACTGCTCCC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.198958	119623	119711	902-902	0.500000	58.4
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	TGAAATCGGTAGACACGCTG	CATTAGCCCATACTGCTCCC	20	20	50.0	55.0	58.0	57.8	0.0	0.0	0.200329	119623	119711	901-901	0.500000	58.4
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	GTGAAATCGGTAGACACGCT	GTAGCAAAGCCCTGGGTAAA	20	20	50.0	50.0	58.0	57.8	0.0	15.6	0.217520	119623	119711	501-501	0.500000	58.3
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	CTCGACGAAATGGGTGGAAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032006	119712	120683	1731-1731	0.500000	59.7
ccsA_p2	ccsA	2	TGAAATCGGTAGACACGCTG	CTCGACGAAATGGGTGGAAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.033376	119712	120683	1730-1730	0.500000	59.7
ccsA_p3	ccsA	3	GTGAAATCGGTAGACACGCT	TTATCGATGGCTTCCCTTGC	20	20	50.0	50.0	58.0	58.0	0.0	8.5	0.040512	119712	120683	1671-1671	0.500000	59.7
ccsA_p4	ccsA	4	TGAAATCGGTAGACACGCTG	TTATCGATGGCTTCCCTTGC	20	20	50.0	50.0	58.0	58.0	0.0	8.5	0.041882	119712	120683	1670-1670	0.500000	59.7
ccsA_p5	ccsA	5	GGCGGCATGGAATCTTCTAA	CTCGACGAAATGGGTGGAAT	20	20	50.0	50.0	58.0	58.0	3.1	0.0	0.056857	119712	120683	1667-1667	0.500000	59.5
ndhD_p1	ndhD	1	AGGTGCAAATTCCGCAATTG	ATCTCTCATGAGCCGATCCA	20	20	45.0	50.0	57.9	58.0	0.0	18.4	0.089972	120860	122362	2318-2318	0.500000	59.3
ndhD_p2	ndhD	2	CAAGGTGCAAATTCCGCAAT	ATCTCTCATGAGCCGATCCA	20	20	45.0	50.0	57.9	58.0	4.2	18.4	0.089972	120860	122362	2320-2320	0.500000	59.3
ndhD_p3	ndhD	3	GAGGCATGGGGGTCATATTG	ATCTCTCATGAGCCGATCCA	20	20	55.0	50.0	58.1	58.0	0.0	18.4	0.091665	120860	122362	2425-2425	0.500000	59.3
ndhD_p4	ndhD	4	AGGTGCAAATTCCGCAATTG	CGTTTCAGAAAACTCCACGC	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.101884	120860	122362	1918-1918	0.500000	59.2
ndhD_p5	ndhD	5	AGGTGCAAATTCCGCAATTG	GTTTCAGAAAACTCCACGCG	20	20	45.0	50.0	57.9	58.0	0.0	3.5	0.101884	120860	122362	1917-1917	0.500000	59.2
ndhD-psaC_p1	ndhD-psaC	1	GCTTCTCCATGAGTGTCTGG	ATCTCTCATGAGCCGATCCA	20	20	55.0	50.0	58.0	58.0	0.0	18.4	0.028161	122363	122479	1237-1237	0.500000	59.8
ndhD-psaC_p2	ndhD-psaC	2	TTCCTCCCCACATAGCTAGG	ATCTCTCATGAGCCGATCCA	20	20	55.0	50.0	57.9	58.0	3.3	18.4	0.109959	122363	122479	987-987	0.500000	59.1
ndhD-psaC_p3	ndhD-psaC	3	GCTTCTCCATGAGTGTCTGG	TGTGAATCTGCCTGTCCAAC	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.125135	122363	122479	926-926	0.500000	59.0
ndhD-psaC_p4	ndhD-psaC	4	GCTTCTCCATGAGTGTCTGG	TGCTTCTGCTCCAAGAACAG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.133140	122363	122479	969-969	0.500000	58.9
ndhD-psaC_p5	ndhD-psaC	5	GCTTCTCCATGAGTGTCTGG	TGATACCTTGGGACGGATGT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.147226	122363	122479	1003-1003	0.500000	58.8
psaC_p1	psaC	1	CGCGTGGAGTTTTCTGAAAC	ATCTCTCATGAGCCGATCCA	20	20	50.0	50.0	58.0	58.0	1.6	18.4	0.025522	122480	122725	421-421	0.500000	59.8
psaC_p2	psaC	2	TCGCGTGGAGTTTTCTGAAA	ATCTCTCATGAGCCGATCCA	20	20	45.0	50.0	58.1	58.0	5.8	18.4	0.064128	122480	122725	422-422	0.500000	59.5
psaC_p3	psaC	3	TTCGCGTGGAGTTTTCTGAA	ATCTCTCATGAGCCGATCCA	20	20	45.0	50.0	58.1	58.0	5.8	18.4	0.064128	122480	122725	423-423	0.500000	59.5
psaC_p4	psaC	4	GGACCAGAAAAACCCGTGAT	ATCTCTCATGAGCCGATCCA	20	20	50.0	50.0	58.1	58.0	0.0	18.4	0.101168	122480	122725	495-495	0.500000	59.2
psaC_p5	psaC	5	TTCCTCCCCACATAGCTAGG	ATCTCTCATGAGCCGATCCA	20	20	55.0	50.0	57.9	58.0	3.3	18.4	0.109959	122480	122725	987-987	0.500000	59.1
ndhG_p1	ndhG	1	TGGATCGGCTCATGAGAGAT	ATCAAATCGCTTTGGGTCGT	20	20	50.0	45.0	58.0	58.2	27.0	11.5	0.185589	123561	124091	1709-1709	0.500000	58.5
ndhG_p2	ndhG	2	TGGATCGGCTCATGAGAGAT	ACAACAGGATAAACCCCACG	20	20	50.0	50.0	58.0	57.8	27.0	0.0	0.201007	123561	124091	1632-1632	0.500000	58.4
ndhG_p3	ndhG	3	TGGATCGGCTCATGAGAGAT	TCTACATCAACCCACACCCA	20	20	50.0	50.0	58.0	58.3	27.0	0.0	0.270879	123561	124091	1396-1396	0.500000	57.8
ndhG_p4	ndhG	4	TGGATCGGCTCATGAGAGAT	ATTCGCCTCAAACAACAGGA	20	20	50.0	45.0	58.0	57.7	27.0	0.0	0.277157	123561	124091	1643-1643	0.500000	57.8
ndhG_p5	ndhG	5	TGGATCGGCTCATGAGAGAT	TTCGCCTCAAACAACAGGAT	20	20	50.0	45.0	58.0	57.7	27.0	0.0	0.277157	123561	124091	1642-1642	0.500000	57.8
ndhI_p1	ndhI	1	GTGCAGCAGCTACAAAATGG	AAAGCAGGTGGAGTCTTTGG	20	20	50.0	50.0	58.0	58.0	1.8	0.0	0.026420	124478	124978	1352-1352	0.500000	59.8
ndhI_p2	ndhI	2	GTGCAGCAGCTACAAAATGG	TACTTGGGTGGGTGGAATCT	20	20	50.0	50.0	58.0	57.9	1.8	0.0	0.067049	124478	124978	1415-1415	0.500000	59.5
ndhI_p3	ndhI	3	GTGCAGCAGCTACAAAATGG	TCTTCCCAACTCCTGTCACT	20	20	50.0	50.0	58.0	57.9	1.8	0.0	0.116456	124478	124978	1169-1169	0.500000	59.1
ndhI_p4	ndhI	4	ACAAACCGTCGAAGTAACCC	AAAGCAGGTGGAGTCTTTGG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.143344	124478	124978	1480-1480	0.500000	58.9
ndhI_p5	ndhI	5	GAAGTAACCCCATCCCCAAC	AAAGCAGGTGGAGTCTTTGG	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.152441	124478	124978	1470-1470	0.500000	58.8
ndhA_p1	ndhA	1	TGCAGCTCGTACTGTTTGTT	AAGCACCAAAGGGAGAGTTG	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.065013	125053	127261	2532-2532	0.500000	59.5
ndhA_p2	ndhA	2	TTGCAGCTCGTACTGTTTGT	AAGCACCAAAGGGAGAGTTG	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.065013	125053	127261	2533-2533	0.500000	59.5
ndhA_p3	ndhA	3	TGCAGCTCGTACTGTTTGTT	TTAGAACGGGTTGAGGGAGT	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.071703	125053	127261	2926-2926	0.500000	59.4
ndhA_p4	ndhA	4	TTGCAGCTCGTACTGTTTGT	TTAGAACGGGTTGAGGGAGT	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.071703	125053	127261	2927-2927	0.500000	59.4
ndhA_p5	ndhA	5	TGCAGCTCGTACTGTTTGTT	GCATTATTGGCGGAGAGGAA	20	20	45.0	50.0	58.1	58.0	8.8	0.0	0.084577	125053	127261	2904-2904	0.500000	59.3
ndhH_p1	ndhH	1	TACGTTGTTGTATCCCTGCG	AAAGAAGAAAGCAGGGGGTC	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.295401	127263	128444	1705-1705	0.500000	57.6
ndhH_p2	ndhH	2	TACGTTGTTGTATCCCTGCG	TTCTGGGAAAACGTCAACGA	20	20	50.0	45.0	58.0	57.7	0.0	9.9	0.313674	127263	128444	1581-1581	0.500000	57.5
ndhH_p3	ndhH	3	TACGTTGTTGTATCCCTGCG	TTGGTCGGTTGGATATTCGG	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.320088	127263	128444	1512-1512	0.500000	57.4
ndhH_p4	ndhH	4	TTGGAGAATTCCCAAAGGGC	AAAGAAGAAAGCAGGGGGTC	20	20	50.0	50.0	58.1	57.7	19.9	0.0	0.357258	127263	128444	1743-1743	0.500000	57.1
ndhH_p5	ndhH	5	TACGTTGTTGTATCCCTGCG	GCAGGGGGTCTGTTGAATTT	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.368363	127263	128444	1695-1695	0.500000	57.1
ndhH-rps15_p1	ndhH-rps15	1	ACTCCCTCAACCCGTTCTAA	GCCGGTTCAGTATGGTAAGG	20	20	50.0	55.0	58.0	58.1	0.0	1.9	0.070342	128445	128551	1443-1443	0.500000	59.4
ndhH-rps15_p2	ndhH-rps15	2	TTCCTAACTGCTCTGGTCCA	GCCGGTTCAGTATGGTAAGG	20	20	50.0	55.0	58.0	58.1	0.0	1.9	0.074710	128445	128551	1097-1097	0.500000	59.4
ndhH-rps15_p3	ndhH-rps15	3	TTCCTCTCCGCCAATAATGC	GCCGGTTCAGTATGGTAAGG	20	20	50.0	55.0	58.0	58.1	0.0	1.9	0.083216	128445	128551	1465-1465	0.500000	59.3
ndhH-rps15_p4	ndhH-rps15	4	TTTATTGCTTCCTCTCCGCC	GCCGGTTCAGTATGGTAAGG	20	20	50.0	55.0	58.0	58.1	0.0	1.9	0.088922	128445	128551	1473-1473	0.500000	59.3
ndhH-rps15_p5	ndhH-rps15	5	TCTGTGCACCAATATCTGCC	GCCGGTTCAGTATGGTAAGG	20	20	50.0	55.0	58.0	58.1	15.7	1.9	0.093221	128445	128551	1211-1211	0.500000	59.3
rps15-ycf1_p1	rps15-ycf1	1	TTCCTAACTGCTCTGGTCCA	GCCGGTTCAGTATGGTAAGG	20	20	50.0	55.0	58.0	58.1	0.0	1.9	0.074710	128825	129220	1097-1097	0.500000	59.4
rps15-ycf1_p2	rps15-ycf1	2	AACACCATGCATTGATGGGT	GCCGGTTCAGTATGGTAAGG	20	20	45.0	55.0	58.1	58.1	8.9	1.9	0.109157	128825	129220	901-901	0.500000	59.1
rps15-ycf1_p3	rps15-ycf1	3	TGCATTGATGGGTGATGAGG	GCCGGTTCAGTATGGTAAGG	20	20	50.0	55.0	57.9	58.1	0.0	1.9	0.112044	128825	129220	894-894	0.500000	59.1
rps15-ycf1_p4	rps15-ycf1	4	TTCCTAACTGCTCTGGTCCA	AAAGGGGCGAAATGAGTCTG	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.197791	128825	129220	1655-1655	0.500000	58.4
rps15-ycf1_p5	rps15-ycf1	5	TTCCTAACTGCTCTGGTCCA	CAAAGGGGCGAAATGAGTCT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.197791	128825	129220	1656-1656	0.500000	58.4
ycf1_p1	ycf1	1	TCTGTGCCAAGGTTTCAGAC	TTCTTGAACGGGCCTTATCG	20	20	50.0	50.0	58.0	58.0	0.0	0.8	0.051223	132473	132592	1191-1191	0.500000	59.6
ycf1_p2	ycf1	2	TCTGTGCCAAGGTTTCAGAC	GGGCCTTATCGTGGAACAAT	20	20	50.0	50.0	58.0	57.9	0.0	0.8	0.085464	132473	132592	1182-1182	0.500000	59.3
ycf1_p3	ycf1	3	AGCTTCCATTCCCCAAACTG	TTCTTGAACGGGCCTTATCG	20	20	50.0	50.0	58.1	58.0	0.0	0.8	0.089674	132473	132592	1287-1287	0.500000	59.3
ycf1_p4	ycf1	4	CAGCTTCCATTCCCCAAACT	TTCTTGAACGGGCCTTATCG	20	20	50.0	50.0	58.1	58.0	0.0	0.8	0.089674	132473	132592	1288-1288	0.500000	59.3
ycf1_p5	ycf1	5	TCAGCTTCCATTCCCCAAAC	TTCTTGAACGGGCCTTATCG	20	20	50.0	50.0	58.1	58.0	0.0	0.8	0.095186	132473	132592	1289-1289	0.500000	59.2