Hotspot

ndhF-chlN

Genome · 121062-121997 · 936 bp

Back to Isoetes

Pi 0.0615

MarkerSeek score 74.70

Primer available yes

Label ndhF-chlN

Feature Metadata

label_name: ndhF-chlN
feature_type: igs
region: Genome
length_bp: 936
variable_sites: 189
pi: 0.0615
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-chlN_p1	GGCGTCGGTATCTCAAGTTT	AGTGTGGGAAAGCTTGGAAG	50.0	50.0	58.0	58.0	1469-1623 bp	0.943396	89.6
ndhF-chlN_p2	GGCGTCGGTATCTCAAGTTT	GTGTGGGAAAGCTTGGAAGA	50.0	50.0	58.0	58.0	1468-1622 bp	0.943396	89.5
ndhF-chlN_p3	GGCGTCGGTATCTCAAGTTT	GGAGCACCTTTTCCTATCGG	50.0	55.0	58.0	58.0	1573-1727 bp	0.943396	89.3
ndhF-chlN_p4	GGCGTCGGTATCTCAAGTTT	GCTCACGCTAATCCATTGGA	50.0	50.0	58.0	58.0	1171-1325 bp	0.943396	89.3
ndhF-chlN_p5	GGCGTCGGTATCTCAAGTTT	TGACTTAGCCATCACTGGGA	50.0	50.0	58.0	58.0	1193-1347 bp	0.924528	88.5