Hotspot

psbM-trnL-CAA

Genome · 86756-87260 · 505 bp

Back to Isoetes

Pi 0.0492

MarkerSeek score 69.40

Primer available yes

Label psbM-trnL-CAA

Feature Metadata

label_name: psbM-trnL-CAA
feature_type: igs
region: Genome
length_bp: 505
variable_sites: 89
pi: 0.0492
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnL-CAA_p1	TGCGAGGATATTTACTTCCA	GTTCGAGTCCTCTTCAAGGC	40.0	55.0	53.9	58.3	532-560 bp	1.0	56.3
psbM-trnL-CAA_p2	ATGCGAGGATATTTACTTCCA	GTTCGAGTCCTCTTCAAGGC	38.1	55.0	54.5	58.3	533-561 bp	1.0	53.5
psbM-trnL-CAA_p3	TGCGAGGATATTTACTTCCAT	GTTCGAGTCCTCTTCAAGGC	38.1	55.0	54.5	58.3	532-560 bp	1.0	53.5
psbM-trnL-CAA_p4	TGCGAGGATATTTACTTCCA	TTCGAGTCCTCTTCAAGGCA	40.0	50.0	53.9	58.7	531-559 bp	1.0	53.3
psbM-trnL-CAA_p5	AATGCGAGGATATTTACTTCCA	GTTCGAGTCCTCTTCAAGGC	36.4	55.0	55.3	58.3	534-562 bp	1.0	52.0