Hotspot

trnK-UUU-chlB

Genome · 62239-63744 · 1506 bp

Back to Isoetes

Pi 0.0354

MarkerSeek score 68.80

Primer available yes

Label trnK-UUU-chlB

Feature Metadata

label_name: trnK-UUU-chlB
feature_type: igs
region: Genome
length_bp: 1506
variable_sites: 241
pi: 0.0354
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-chlB_p1	CTCGTTCTTCTGGTTCCACC	GGGGATTCGTGTGTGTTGTA	55.0	50.0	58.2	57.8	2296-2346 bp	0.509434	69.4
trnK-UUU-chlB_p2	CTCGTTCTTCTGGTTCCACC	GATGGGGATTCGTGTGTGTT	55.0	50.0	58.2	58.2	2313-2349 bp	0.490566	66.2
trnK-UUU-chlB_p3	ACTGATCGCTTACCTGACCT	GGGGATTCGTGTGTGTTGTA	50.0	50.0	58.1	57.8	2550-2594 bp	0.113208	45.4
trnK-UUU-chlB_p4	ACTGATCGCTTACCTGACCT	GATGGGGATTCGTGTGTGTT	50.0	50.0	58.1	58.2	2553-2597 bp	0.113208	45.3
trnK-UUU-chlB_p5	ACTGATCGCTTACCTGACCT	GAGAGATGGGGATTCGTGTG	50.0	55.0	58.1	57.8	2557-2584 bp	0.09434	43.9