Hotspot

trnT-GGU-trnE-UUC

Genome · 55784-57098 · 1315 bp

Back to Isoetes

Pi 0.0385

MarkerSeek score 72.40

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: Genome
length_bp: 1315
variable_sites: 201
pi: 0.0385
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAACCGATGACTTACGCCTT	GAGATGTCCTGGGCCTCTAG	50.0	60.0	58.0	58.7	1399-1685 bp	1.0	86.6
trnT-GGU-trnE-UUC_p2	CCGATGACTTACGCCTTACC	GAGATGTCCTGGGCCTCTAG	55.0	60.0	58.2	58.7	1396-1682 bp	1.0	85.5
trnT-GGU-trnE-UUC_p3	CCATGGCGTTACTCTACCAC	GAGATGTCCTGGGCCTCTAG	55.0	60.0	58.1	58.7	1378-1664 bp	0.981132	85.4
trnT-GGU-trnE-UUC_p4	TGGCGTTACTCTACCACTGA	GAGATGTCCTGGGCCTCTAG	50.0	60.0	58.1	58.7	1375-1661 bp	0.981132	85.3
trnT-GGU-trnE-UUC_p5	TTGAACCGATGACTTACGCC	GAGATGTCCTGGGCCTCTAG	50.0	60.0	58.3	58.7	1401-1687 bp	1.0	84.4