Hotspot

trnF-GAA-trnL-UAA

Genome · 39261-39770 · 510 bp

Back to Isoetes

Pi 0.0407

MarkerSeek score 67.00

Primer available yes

Label trnF-GAA-trnL-UAA

Feature Metadata

label_name: trnF-GAA-trnL-UAA
feature_type: igs
region: Genome
length_bp: 510
variable_sites: 82
pi: 0.0407
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnL-UAA_p1	TTTGAACTGGTGACACGAGG	ATCCGTTGGCTTTATAGGCC	50.0	50.0	58.1	57.7	575-641 bp	1.0	89.4
trnF-GAA-trnL-UAA_p2	TCTACCGACTGAGCTATCCC	ATCCGTTGGCTTTATAGGCC	55.0	50.0	57.7	57.7	539-605 bp	1.0	87.6
trnF-GAA-trnL-UAA_p3	GAACTGGTGACACGAGGATT	ATCCGTTGGCTTTATAGGCC	50.0	50.0	57.5	57.7	572-638 bp	1.0	86.2
trnF-GAA-trnL-UAA_p4	TTCAGTCCTCTGCTCTACCG	ATCCGTTGGCTTTATAGGCC	55.0	50.0	58.5	57.7	552-618 bp	1.0	85.5
trnF-GAA-trnL-UAA_p5	TAGTATGCATGCCAGGAACC	ATCCGTTGGCTTTATAGGCC	50.0	50.0	57.4	57.7	639-640 bp	0.113208	39.4