Hotspot

trnV-UAC-ndhC

Genome · 36129-37075 · 947 bp

Back to Isoetes

Pi 0.0299

MarkerSeek score 70.00

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: Genome
length_bp: 947
variable_sites: 136
pi: 0.0299
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	GGTTCGAATCCGTATAGCCC	CAGGGGAATCAGGCTAGCTA	55.0	55.0	57.9	58.3	843-1062 bp	1.0	89.2
trnV-UAC-ndhC_p2	CCGAGAATGTCTACGGTTCG	CAGGGGAATCAGGCTAGCTA	55.0	55.0	58.2	58.3	857-1076 bp	1.0	88.4
trnV-UAC-ndhC_p3	CGGTTCGAATCCGTATAGCC	CAGGGGAATCAGGCTAGCTA	55.0	55.0	58.3	58.3	844-1063 bp	1.0	87.3
trnV-UAC-ndhC_p4	GGTTCGAATCCGTATAGCCC	AGGGGAATCAGGCTAGCTAT	55.0	50.0	57.9	57.3	842-1061 bp	1.0	85.5
trnV-UAC-ndhC_p5	AGCTGTTTCACCGAGAATGT	CAGGGGAATCAGGCTAGCTA	45.0	55.0	57.4	58.3	867-1086 bp	1.0	85.4