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psaI-trnR-CCG

Genome · 28991-30684 · 1694 bp

Back to Isoetes

Pi 0.0336

MarkerSeek score 68.60

Primer available yes

Label psaI-trnR-CCG

Feature Metadata

label_name: psaI-trnR-CCG
feature_type: igs
region: Genome
length_bp: 1694
variable_sites: 230
pi: 0.0336
unique_haplotype_count: 40
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-trnR-CCG_p1	TCGTTGCGGATGAAGAAACA	CATTCACTCCGGTACTGTGG	45.0	55.0	58.1	58.0	2494-2719 bp	0.698113	78.8
psaI-trnR-CCG_p2	TTGAACTCGTTGCGGATGAA	CATTCACTCCGGTACTGTGG	45.0	55.0	58.1	58.0	2500-2725 bp	0.698113	78.8
psaI-trnR-CCG_p3	TTTGAACTCGTTGCGGATGA	CATTCACTCCGGTACTGTGG	45.0	55.0	58.1	58.0	2501-2726 bp	0.698113	78.8
psaI-trnR-CCG_p4	CCATTCGGATTGCCAATTCG	CATTCACTCCGGTACTGTGG	50.0	55.0	57.9	58.0	2782-2999 bp	0.603774	75.0
psaI-trnR-CCG_p5	TAAGCCATTCGGATTGCCAA	CATTCACTCCGGTACTGTGG	45.0	55.0	57.9	58.0	2786-2998 bp	0.566038	73.4