Hotspot

psbJ-petA

Genome · 23747-25071 · 1325 bp

Back to Isoetes

Pi 0.0373

MarkerSeek score 72.80

Primer available yes

Label psbJ-petA

Feature Metadata

label_name: psbJ-petA
feature_type: igs
region: Genome
length_bp: 1325
variable_sites: 205
pi: 0.0373
unique_haplotype_count: 43
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-petA_p1	ATGGCCCATACTCCGGATTA	TCCTCCTGATCGTATTCCCC	50.0	55.0	58.0	58.0	1967-2062 bp	0.981132	90.8
psbJ-petA_p2	TACGGTTTTCTCCCCAGGAT	TCCTCCTGATCGTATTCCCC	50.0	55.0	58.0	58.0	2370-2465 bp	0.981132	90.8
psbJ-petA_p3	CCCATACTCCGGATTAGGGT	CGTCCGGTGGTTGATATTGT	55.0	50.0	58.0	58.0	1614-1695 bp	0.433962	67.5
psbJ-petA_p4	CCCATACTCCGGATTAGGGT	TCTTTCACCAGACCCTGCTA	55.0	50.0	58.0	58.0	1825-1906 bp	0.433962	66.8
psbJ-petA_p5	CCCATACTCCGGATTAGGGT	TCCTCCTGATCGTATTCCCC	55.0	55.0	58.0	58.0	1963-2044 bp	0.433962	66.3