primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbB-clpP_p1	psbB-clpP	1	ATACTCCAACCACCCCATGA	AGTCGCACTGTACGTCAATC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041649	13942	15056	993-1487	1.000000	91.7
psbB-clpP_p2	psbB-clpP	2	TGATACTCCAACCACCCCAT	AGTCGCACTGTACGTCAATC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.041649	13942	15056	995-1489	1.000000	91.7
psbB-clpP_p3	psbB-clpP	3	GGTTGTGCTTCTCCAGTCAA	AGTCGCACTGTACGTCAATC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054140	13942	15056	1293-1787	1.000000	91.5
psbB-clpP_p4	psbB-clpP	4	TTGTGCTTCTCCAGTCAACC	AGTCGCACTGTACGTCAATC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054140	13942	15056	1291-1785	1.000000	91.5
psbB-clpP_p5	psbB-clpP	5	AGAAACTAACGCTGTGTGCA	AGTCGCACTGTACGTCAATC	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.073354	13942	15056	847-1341	1.000000	91.4
psaJ-trnP-UGG_p1	psaJ-trnP-UGG	1	AGGCGCTGTGGAAAGATATG	CGAACTGAACTAAGAGCGCT	20	20	50.0	50.0	58.0	58.0	7.6	11.3	0.056986	19712	20380	924-991	1.000000	91.5
psaJ-trnP-UGG_p2	psaJ-trnP-UGG	2	CAGGCGCTGTGGAAAGATAT	CGAACTGAACTAAGAGCGCT	20	20	50.0	50.0	58.0	58.0	7.6	11.3	0.056986	19712	20380	925-992	1.000000	91.5
psaJ-trnP-UGG_p3	psaJ-trnP-UGG	3	CACGAACCTCCCCAATACTG	CGAACTGAACTAAGAGCGCT	20	20	55.0	50.0	58.0	58.0	0.0	11.3	0.040203	19712	20380	1512-1578	0.924528	88.7
psaJ-trnP-UGG_p4	psaJ-trnP-UGG	4	CACGAACCTCCCCAATACTG	GGAGACCTACGTTCTACCGA	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.054014	19712	20380	1529-1595	0.924528	88.5
psaJ-trnP-UGG_p5	psaJ-trnP-UGG	5	TCACGAACCTCCCCAATACT	CGAACTGAACTAAGAGCGCT	20	20	50.0	50.0	58.1	58.0	0.0	11.3	0.069043	19712	20380	1513-1579	0.924528	88.4
petL-psbE_p1	petL-psbE	1	AAAGCTGGAGTTAGAGCAGC	CTCTCCCGTATTCCCAGACA	20	20	50.0	55.0	57.9	58.2	0.0	0.0	0.329815	21164	22978	1420-1892	1.000000	89.4
petL-psbE_p2	petL-psbE	2	AAAGCTGGAGTTAGAGCAGC	TCTCCCGTATTCCCAGACAT	20	20	50.0	50.0	57.9	57.5	0.0	0.0	0.578292	21164	22978	1419-1891	1.000000	87.4
petL-psbE_p3	petL-psbE	3	CTGGAGTTAGAGCAGCCAAT	CTCTCCCGTATTCCCAGACA	20	20	50.0	55.0	57.3	58.2	0.0	0.0	0.929677	21164	22978	1416-1888	1.000000	84.6
petL-psbE_p4	petL-psbE	4	AAAGCTGGAGTTAGAGCAGC	AATAATGTCGGCGAAAGGGC	20	20	50.0	50.0	57.9	59.0	0.0	12.9	1.084509	21164	22978	1441-1913	1.000000	83.3
petL-psbE_p5	petL-psbE	5	CTGGAGTTAGAGCAGCCAAT	TCTCCCGTATTCCCAGACAT	20	20	50.0	50.0	57.3	57.5	0.0	0.0	1.178154	21164	22978	1415-1887	1.000000	82.6
psbJ-petA_p1	psbJ-petA	1	ATGGCCCATACTCCGGATTA	TCCTCCTGATCGTATTCCCC	20	20	50.0	55.0	58.0	58.0	3.6	0.0	0.049985	23747	25071	1967-2062	0.981132	90.8
psbJ-petA_p2	psbJ-petA	2	TACGGTTTTCTCCCCAGGAT	TCCTCCTGATCGTATTCCCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.049989	23747	25071	2370-2465	0.981132	90.8
psbJ-petA_p3	psbJ-petA	3	CCCATACTCCGGATTAGGGT	CGTCCGGTGGTTGATATTGT	20	20	55.0	50.0	58.0	58.0	25.4	0.0	0.044178	23747	25071	1614-1695	0.433962	67.5
psbJ-petA_p4	psbJ-petA	4	CCCATACTCCGGATTAGGGT	TCTTTCACCAGACCCTGCTA	20	20	55.0	50.0	58.0	58.0	25.4	0.0	0.049811	23747	25071	1825-1906	0.433962	66.8
psbJ-petA_p5	psbJ-petA	5	CCCATACTCCGGATTAGGGT	TCCTCCTGATCGTATTCCCC	20	20	55.0	55.0	58.0	58.0	25.4	0.0	0.036758	23747	25071	1963-2044	0.433962	66.3
psaI-trnR-CCG_p1	psaI-trnR-CCG	1	TCGTTGCGGATGAAGAAACA	CATTCACTCCGGTACTGTGG	20	20	45.0	55.0	58.1	58.0	0.0	0.0	0.137757	28991	30684	2494-2719	0.698113	78.8
psaI-trnR-CCG_p2	psaI-trnR-CCG	2	TTGAACTCGTTGCGGATGAA	CATTCACTCCGGTACTGTGG	20	20	45.0	55.0	58.1	58.0	0.0	0.0	0.137757	28991	30684	2500-2725	0.698113	78.8
psaI-trnR-CCG_p3	psaI-trnR-CCG	3	TTTGAACTCGTTGCGGATGA	CATTCACTCCGGTACTGTGG	20	20	45.0	55.0	58.1	58.0	0.0	0.0	0.137757	28991	30684	2501-2726	0.698113	78.8
psaI-trnR-CCG_p4	psaI-trnR-CCG	4	CCATTCGGATTGCCAATTCG	CATTCACTCCGGTACTGTGG	20	20	50.0	55.0	57.9	58.0	12.0	0.0	0.134156	28991	30684	2782-2999	0.603774	75.0
psaI-trnR-CCG_p5	psaI-trnR-CCG	5	TAAGCCATTCGGATTGCCAA	CATTCACTCCGGTACTGTGG	20	20	45.0	55.0	57.9	58.0	0.0	0.0	0.146443	28991	30684	2786-2998	0.566038	73.4
rbcL-atpB_p1	rbcL-atpB	1	TCTCTCCAACGCATAAACGG	CGTCCAAGAGTAGCTTCACC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.006913	32373	33203	1787-1833	1.000000	91.9
rbcL-atpB_p2	rbcL-atpB	2	TGACCCTTAATCTCACCCGT	CGTCCAAGAGTAGCTTCACC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.055346	32373	33203	1853-1899	1.000000	91.6
rbcL-atpB_p3	rbcL-atpB	3	ACCTTCCTCGAACAGATCCA	CGTCCAAGAGTAGCTTCACC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.063746	32373	33203	1473-1519	1.000000	91.5
rbcL-atpB_p4	rbcL-atpB	4	GCTTTGAATCCAACACTCGC	CGTCCAAGAGTAGCTTCACC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.033228	32373	33203	1184-1230	0.830189	84.9
rbcL-atpB_p5	rbcL-atpB	5	CGGTCTCTCCAACGCATAAA	CGTCCAAGAGTAGCTTCACC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.006913	32373	33203	1790-1836	0.679245	79.1
trnV-UAC-ndhC_p1	trnV-UAC-ndhC	1	GGTTCGAATCCGTATAGCCC	CAGGGGAATCAGGCTAGCTA	20	20	55.0	55.0	57.9	58.3	0.0	15.7	0.353945	36129	37075	843-1062	1.000000	89.2
trnV-UAC-ndhC_p2	trnV-UAC-ndhC	2	CCGAGAATGTCTACGGTTCG	CAGGGGAATCAGGCTAGCTA	20	20	55.0	55.0	58.2	58.3	0.0	15.7	0.454138	36129	37075	857-1076	1.000000	88.4
trnV-UAC-ndhC_p3	trnV-UAC-ndhC	3	CGGTTCGAATCCGTATAGCC	CAGGGGAATCAGGCTAGCTA	20	20	55.0	55.0	58.3	58.3	22.8	15.7	0.583327	36129	37075	844-1063	1.000000	87.3
trnV-UAC-ndhC_p4	trnV-UAC-ndhC	4	GGTTCGAATCCGTATAGCCC	AGGGGAATCAGGCTAGCTAT	20	20	55.0	50.0	57.9	57.3	0.0	15.7	0.807318	36129	37075	842-1061	1.000000	85.5
trnV-UAC-ndhC_p5	trnV-UAC-ndhC	5	AGCTGTTTCACCGAGAATGT	CAGGGGAATCAGGCTAGCTA	20	20	45.0	55.0	57.4	58.3	0.0	15.7	0.828796	36129	37075	867-1086	1.000000	85.4
trnF-GAA-trnL-UAA_p1	trnF-GAA-trnL-UAA	1	TTTGAACTGGTGACACGAGG	ATCCGTTGGCTTTATAGGCC	20	20	50.0	50.0	58.1	57.7	20.1	0.0	0.323840	39261	39770	575-641	1.000000	89.4
trnF-GAA-trnL-UAA_p2	trnF-GAA-trnL-UAA	2	TCTACCGACTGAGCTATCCC	ATCCGTTGGCTTTATAGGCC	20	20	55.0	50.0	57.7	57.7	0.0	0.0	0.545482	39261	39770	539-605	1.000000	87.6
trnF-GAA-trnL-UAA_p3	trnF-GAA-trnL-UAA	3	GAACTGGTGACACGAGGATT	ATCCGTTGGCTTTATAGGCC	20	20	50.0	50.0	57.5	57.7	20.1	0.0	0.729310	39261	39770	572-638	1.000000	86.2
trnF-GAA-trnL-UAA_p4	trnF-GAA-trnL-UAA	4	TTCAGTCCTCTGCTCTACCG	ATCCGTTGGCTTTATAGGCC	20	20	55.0	50.0	58.5	57.7	0.0	0.0	0.807210	39261	39770	552-618	1.000000	85.5
trnF-GAA-trnL-UAA_p5	trnF-GAA-trnL-UAA	5	TAGTATGCATGCCAGGAACC	ATCCGTTGGCTTTATAGGCC	20	20	50.0	50.0	57.4	57.7	16.7	0.0	0.910424	39261	39770	639-640	0.113208	39.4
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	ACTTTCCAATGGCTATAGTCA	GTGGTAGAGTAACGCCATGG	21	20	38.1	55.0	54.3	58.1	10.0	7.6	4.746057	54379	55711	1413-1512	1.000000	54.0
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	ACTTTCCAATGGCTATAGTCA	TCAGTGGTAGAGTAACGCCA	21	20	38.1	50.0	54.3	58.1	10.0	0.0	4.768211	54379	55711	1416-1515	1.000000	53.9
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	AACTTTCCAATGGCTATAGTCA	GTGGTAGAGTAACGCCATGG	22	20	36.4	55.0	55.1	58.1	10.0	7.6	4.919347	54379	55711	1414-1513	1.000000	52.6
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	AACTTTCCAATGGCTATAGTCA	TCAGTGGTAGAGTAACGCCA	22	20	36.4	50.0	55.1	58.1	10.0	0.0	4.941501	54379	55711	1417-1516	1.000000	52.5
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	ACTTTCCAATGGCTATAGTCA	GGCGTAAGTCATCGGTTCAA	21	20	38.1	50.0	54.3	58.3	10.0	0.0	4.964795	54379	55711	1390-1489	1.000000	52.3
trnT-GGU-trnE-UUC_p1	trnT-GGU-trnE-UUC	1	GAACCGATGACTTACGCCTT	GAGATGTCCTGGGCCTCTAG	20	20	50.0	60.0	58.0	58.7	0.0	6.1	0.668852	55784	57098	1399-1685	1.000000	86.6
trnT-GGU-trnE-UUC_p2	trnT-GGU-trnE-UUC	2	CCGATGACTTACGCCTTACC	GAGATGTCCTGGGCCTCTAG	20	20	55.0	60.0	58.2	58.7	0.0	6.1	0.813785	55784	57098	1396-1682	1.000000	85.5
trnT-GGU-trnE-UUC_p3	trnT-GGU-trnE-UUC	3	CCATGGCGTTACTCTACCAC	GAGATGTCCTGGGCCTCTAG	20	20	55.0	60.0	58.1	58.7	0.0	6.1	0.726949	55784	57098	1378-1664	0.981132	85.4
trnT-GGU-trnE-UUC_p4	trnT-GGU-trnE-UUC	4	TGGCGTTACTCTACCACTGA	GAGATGTCCTGGGCCTCTAG	20	20	50.0	60.0	58.1	58.7	0.0	6.1	0.749104	55784	57098	1375-1661	0.981132	85.3
trnT-GGU-trnE-UUC_p5	trnT-GGU-trnE-UUC	5	TTGAACCGATGACTTACGCC	GAGATGTCCTGGGCCTCTAG	20	20	50.0	60.0	58.3	58.7	0.0	6.1	0.945688	55784	57098	1401-1687	1.000000	84.4
trnK-UUU-chlB_p1	trnK-UUU-chlB	1	CTCGTTCTTCTGGTTCCACC	GGGGATTCGTGTGTGTTGTA	20	20	55.0	50.0	58.2	57.8	0.0	0.0	0.375827	62239	63744	2296-2346	0.509434	69.4
trnK-UUU-chlB_p2	trnK-UUU-chlB	2	CTCGTTCTTCTGGTTCCACC	GATGGGGATTCGTGTGTGTT	20	20	55.0	50.0	58.2	58.2	0.0	0.0	0.381759	62239	63744	2313-2349	0.490566	66.2
trnK-UUU-chlB_p3	trnK-UUU-chlB	3	ACTGATCGCTTACCTGACCT	GGGGATTCGTGTGTGTTGTA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.318888	62239	63744	2550-2594	0.113208	45.4
trnK-UUU-chlB_p4	trnK-UUU-chlB	4	ACTGATCGCTTACCTGACCT	GATGGGGATTCGTGTGTGTT	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.324821	62239	63744	2553-2597	0.113208	45.3
trnK-UUU-chlB_p5	trnK-UUU-chlB	5	ACTGATCGCTTACCTGACCT	GAGAGATGGGGATTCGTGTG	20	20	50.0	55.0	58.1	57.8	0.0	0.0	0.377252	62239	63744	2557-2584	0.094340	43.9
psbK-psbI_p1	psbK-psbI	1	CCTCCGTGAGTTTCCGATAA	ACCACTGTGTAAACGAGCAG	20	20	50.0	50.0	57.0	58.1	0.0	7.1	1.087601	66114	66495	396-502	0.981132	82.5
psbK-psbI_p2	psbK-psbI	2	CCTCCGTGAGTTTCCGATAA	GACCACTGTGTAAACGAGCA	20	20	50.0	50.0	57.0	58.1	0.0	7.1	1.092790	66114	66495	397-503	0.981132	82.5
psbK-psbI_p3	psbK-psbI	3	CCTCCGTGAGTTTCCGATAA	TGACCACTGTGTAAACGAGC	20	20	50.0	50.0	57.0	58.1	0.0	7.1	1.092790	66114	66495	398-504	0.981132	82.5
psbK-psbI_p4	psbK-psbI	4	CCTCCGTGAGTTTCCGATAA	TGTGTAAACGAGCAGCTTGA	20	20	50.0	45.0	57.0	57.8	0.0	17.3	1.186222	66114	66495	391-497	0.981132	81.8
psbK-psbI_p5	psbK-psbI	5	CCTCCGTGAGTTTCCGATAA	ACTGTGTAAACGAGCAGCTT	20	20	50.0	45.0	57.0	57.8	0.0	0.0	1.193959	66114	66495	393-499	0.981132	81.7
psbM-trnL-CAA_p1	psbM-trnL-CAA	1	TGCGAGGATATTTACTTCCA	GTTCGAGTCCTCTTCAAGGC	20	20	40.0	55.0	53.9	58.3	0.0	0.0	4.431310	86756	87260	532-560	1.000000	56.3
psbM-trnL-CAA_p2	psbM-trnL-CAA	2	ATGCGAGGATATTTACTTCCA	GTTCGAGTCCTCTTCAAGGC	21	20	38.1	55.0	54.5	58.3	0.0	0.0	4.781692	86756	87260	533-561	1.000000	53.5
psbM-trnL-CAA_p3	psbM-trnL-CAA	3	TGCGAGGATATTTACTTCCAT	GTTCGAGTCCTCTTCAAGGC	21	20	38.1	55.0	54.5	58.3	0.0	0.0	4.781692	86756	87260	532-560	1.000000	53.5
psbM-trnL-CAA_p4	psbM-trnL-CAA	4	TGCGAGGATATTTACTTCCA	TTCGAGTCCTCTTCAAGGCA	20	20	40.0	50.0	53.9	58.7	0.0	0.0	4.809196	86756	87260	531-559	1.000000	53.3
psbM-trnL-CAA_p5	psbM-trnL-CAA	5	AATGCGAGGATATTTACTTCCA	GTTCGAGTCCTCTTCAAGGC	22	20	36.4	55.0	55.3	58.3	0.0	0.0	4.969461	86756	87260	534-562	1.000000	52.0
ccsA-trnL-UAG_p1	ccsA-trnL-UAG	1	TGTTCCAAGGTTCTGAGTATCG	AAGCATCTCGGTTCGAATCC	22	20	45.5	50.0	57.8	58.1	0.0	0.0	2.246922	116972	117158	227-272	0.962264	72.5
ccsA-trnL-UAG_p2	ccsA-trnL-UAG	2	GTTCCAAGGTTCTGAGTATCGT	AAGCATCTCGGTTCGAATCC	22	20	45.5	50.0	57.8	58.1	0.0	0.0	2.248314	116972	117158	226-271	0.962264	72.5
ccsA-trnL-UAG_p3	ccsA-trnL-UAG	3	TGTTCCAAGGTTCTGAGTATCG	GCTAAAGCATCTCGGTTCGA	22	20	45.5	50.0	57.8	58.1	0.0	0.0	2.265676	116972	117158	231-276	0.962264	72.4
ccsA-trnL-UAG_p4	ccsA-trnL-UAG	4	GTTCCAAGGTTCTGAGTATCGT	GCTAAAGCATCTCGGTTCGA	22	20	45.5	50.0	57.8	58.1	0.0	0.0	2.267069	116972	117158	230-275	0.962264	72.4
ccsA-trnL-UAG_p5	ccsA-trnL-UAG	5	TGTTCCAAGGTTCTGAGTATCG	TAGACACGCTGCTCTTAGGA	22	20	45.5	50.0	57.8	57.9	0.0	0.0	2.312524	116972	117158	257-302	0.962264	72.0
trnP-GGG-rpl32_p1	trnP-GGG-rpl32	1	ATAGGGAAGGGTGACGGAAA	GGAGGTGCAGTAGTGAAAGG	20	20	50.0	55.0	58.0	57.9	0.0	0.4	0.139472	117438	117798	1367-1484	0.622642	75.8
trnP-GGG-rpl32_p2	trnP-GGG-rpl32	2	ATAGGGAAGGGTGACGGAAA	AAGAATTCTGCAGTCCCGTC	20	20	50.0	50.0	58.0	57.9	0.0	14.7	0.139472	117438	117798	1351-1464	0.452830	69.0
trnP-GGG-rpl32_p3	trnP-GGG-rpl32	3	ATAGGGAAGGGTGACGGAAA	GAAGAATTCTGCAGTCCCGT	20	20	50.0	50.0	58.0	57.9	0.0	14.7	0.139472	117438	117798	1352-1465	0.452830	69.0
trnP-GGG-rpl32_p4	trnP-GGG-rpl32	4	ATAGGGAAGGGTGACGGAAA	GAATTCTGCAGTCCCGTCTT	20	20	50.0	50.0	58.0	57.9	0.0	14.7	0.139472	117438	117798	1349-1462	0.452830	69.0
trnP-GGG-rpl32_p5	trnP-GGG-rpl32	5	ATAGGGAAGGGTGACGGAAA	GGGAGGTGCAGTAGTGAAAG	20	20	50.0	55.0	58.0	57.9	0.0	0.4	0.139472	117438	117798	1372-1485	0.452830	69.0
ndhF-chlN_p1	ndhF-chlN	1	GGCGTCGGTATCTCAAGTTT	AGTGTGGGAAAGCTTGGAAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.019088	121062	121997	1469-1623	0.943396	89.6
ndhF-chlN_p2	ndhF-chlN	2	GGCGTCGGTATCTCAAGTTT	GTGTGGGAAAGCTTGGAAGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024714	121062	121997	1468-1622	0.943396	89.5
ndhF-chlN_p3	ndhF-chlN	3	GGCGTCGGTATCTCAAGTTT	GGAGCACCTTTTCCTATCGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.051044	121062	121997	1573-1727	0.943396	89.3
ndhF-chlN_p4	ndhF-chlN	4	GGCGTCGGTATCTCAAGTTT	GCTCACGCTAATCCATTGGA	20	20	50.0	50.0	58.0	58.0	0.0	8.1	0.052412	121062	121997	1171-1325	0.943396	89.3
ndhF-chlN_p5	ndhF-chlN	5	GGCGTCGGTATCTCAAGTTT	TGACTTAGCCATCACTGGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.054761	121062	121997	1193-1347	0.924528	88.5
ycf2_p1	ycf2	1	ACGTCAAGCCATTCTTCGTA	GGCGACCGATACAGCTTTTA	20	20	45.0	50.0	57.3	58.1	0.0	0.0	0.805576	124903	125022	759-822	0.773585	76.5
ycf2_p2	ycf2	2	TACGTCAAGCCATTCTTCGT	GGCGACCGATACAGCTTTTA	20	20	45.0	50.0	57.3	58.1	0.0	0.0	0.805576	124903	125022	760-823	0.773585	76.5
ycf2_p3	ycf2	3	ACGTCAAGCCATTCTTCGTA	TTTTTCATACAGGAGGGCGG	20	20	45.0	50.0	57.3	57.9	0.0	0.0	0.858927	124903	125022	795-858	0.773585	76.1
ycf2_p4	ycf2	4	TACGTCAAGCCATTCTTCGT	TTTTTCATACAGGAGGGCGG	20	20	45.0	50.0	57.3	57.9	0.0	0.0	0.858927	124903	125022	796-859	0.773585	76.1
ycf2_p5	ycf2	5	ACGTCAAGCCATTCTTCGTA	AGATCCCTCCATCGACGAAA	20	20	45.0	50.0	57.3	58.2	0.0	0.0	0.959482	124903	125022	490-532	0.377358	59.4