Hotspot

ndhA

SSC · 118294-120416 · 2123 bp

Pi 0.00524

MarkerSeek score 57.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2123
variable_sites: 135
pi: 0.00524
unique_haplotype_count: 67
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AGAACCGAGTTAGCATAGGGA	CCGGGGTTTATCAACTTGCA	47.6	50.0	58.0	58.5	2321-2353 bp	1.0	74.6
ndhA_p2	CCGAGTTAGCATAGGGAACA	CCGGGGTTTATCAACTTGCA	50.0	50.0	56.7	58.5	2317-2349 bp	1.0	72.6
ndhA_p3	ACCGAGTTAGCATAGGGAAC	CCGGGGTTTATCAACTTGCA	50.0	50.0	56.7	58.5	2318-2350 bp	1.0	72.6
ndhA_p4	AGAACCGAGTTAGCATAGGGA	ACTTGCAAATTCTTCCGCAG	47.6	45.0	58.0	57.3	2308-2340 bp	1.0	72.5
ndhA_p5	AGAACCGAGTTAGCATAGGGA	CTTGCAAATTCTTCCGCAGT	47.6	45.0	58.0	57.3	2307-2339 bp	1.0	72.5