Hotspot

ycf3-trnS-GGA

LSC · 44086-44948 · 863 bp

Back to Isodon

Pi 0.00596
MarkerSeek score 57.10
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
863
variable_sites
55
pi
0.00596
unique_haplotype_count
39
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TGCCTCCTTTTCTCCTGAAG CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 929-1042 bp 1.0 80.7
ycf3-trnS-GGA_p2 GCCTCCTTTTCTCCTGAAGT CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 928-1041 bp 1.0 80.7
ycf3-trnS-GGA_p3 TGCCTCCTTTTCTCCTGAAG AAGTGAAGGTGCGGAAAGAG 50.0 50.0 57.1 58.1 960-1073 bp 1.0 80.4
ycf3-trnS-GGA_p4 GCCTCCTTTTCTCCTGAAGT AAGTGAAGGTGCGGAAAGAG 50.0 50.0 57.1 58.1 959-1072 bp 1.0 80.4
ycf3-trnS-GGA_p5 TGCCTCCTTTTCTCCTGAAG TTCCAATGCTACGCCTTGAA 50.0 45.0 57.1 57.8 900-1013 bp 1.0 80.1