Hotspot

psaA-ycf3

LSC · 41364-42137 · 774 bp

Pi 0.0062

MarkerSeek score 59.20

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 42
pi: 0.0062
unique_haplotype_count: 60
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	859-915 bp	1.0	86.1
psaA-ycf3_p2	GAAAAATGACCCGGTTTGGC	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.2	57.9	877-933 bp	1.0	85.3
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.9	57.7	932-988 bp	1.0	84.6
psaA-ycf3_p4	GAAAAATGACCCGGTTTGGC	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.2	57.7	950-1006 bp	1.0	83.8
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	CGGAGGCTTGGTTTGATCAA	50.0	50.0	57.9	58.5	940-996 bp	1.0	83.1