Hotspot

trnH-GUG-psbA

LSC · 86-392 · 307 bp

Back to Isodon

Pi 0.0189
MarkerSeek score 54.20
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
307
variable_sites
38
pi
0.0189
unique_haplotype_count
42
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TCAGTGCTATGCATGGTTCC 55.0 50.0 58.1 58.0 680-769 bp 1.0 88.3
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG CAGTGCTATGCATGGTTCCT 55.0 50.0 58.1 58.0 679-768 bp 1.0 88.3
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA TCAGTGCTATGCATGGTTCC 50.0 50.0 57.9 58.0 694-783 bp 1.0 88.2
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC TCAGTGCTATGCATGGTTCC 50.0 50.0 57.9 58.0 693-782 bp 1.0 88.2
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC TCAGTGCTATGCATGGTTCC 50.0 50.0 57.9 58.0 695-784 bp 0.988372 87.6