Hotspot

ndhD

SSC · 109510-111012 · 1503 bp

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Pi 0.00209

MarkerSeek score 53.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 9
pi: 0.00209
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGGAATATAGAGCGGACTGGT	GGGCTTTTCTGGTCAAAACG	47.6	50.0	58.0	57.9	1582 bp	1.0	71.1
ndhD_p2	TGGAATATAGAGCGGACTGGT	TTTACCGAAGAAGCCTGTGC	47.6	50.0	58.0	58.5	1619 bp	1.0	68.3
ndhD_p3	CCACTCTGGAATATAGAGCGG	GGGCTTTTCTGGTCAAAACG	52.4	50.0	57.4	57.9	1588 bp	1.0	66.7
ndhD_p4	CTGGAATATAGAGCGGACTGG	GGGCTTTTCTGGTCAAAACG	52.4	50.0	57.4	57.9	1583 bp	1.0	66.7
ndhD_p5	TGGAATATAGAGCGGACTGGT	GGCTTTTCTGGTCAAAACGT	47.6	45.0	58.0	57.1	1581 bp	1.0	65.2