Hotspot

ccsA

SSC · 108364-109329 · 966 bp

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Pi 0.00325
MarkerSeek score 36.80
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
966
variable_sites
7
pi
0.00325
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 CTCAAGCATCTCGGTTCGAA GGGTTCTCAAATCAAACAAA 50.0 35.0 58.0 52.1 1131 bp 1.0 40.5
ccsA_p2 CTCAAGCATCTCGGTTCGAA GGGTTCTCAAATCAAACAAAA 50.0 33.3 58.0 53.1 1131 bp 1.0 40.5
ccsA_p3 AAGCATCTCGGTTCGAATCC GGGTTCTCAAATCAAACAAA 50.0 35.0 58.1 52.1 1128 bp 1.0 40.5
ccsA_p4 AAGCATCTCGGTTCGAATCC GGGTTCTCAAATCAAACAAAA 50.0 33.3 58.1 53.1 1128 bp 1.0 40.5
ccsA_p5 CTCAAGCATCTCGGTTCGAA GGGTTCTCAAATCAAACAAAAA 50.0 31.8 58.0 53.9 1131 bp 1.0 40.5