Hotspot

trnV-GAC-rps12

IRa · 127283-129022 · 1740 bp

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Pi 0.0012

MarkerSeek score 55.20

Primer available yes

Label trnV-GAC-rps12

Feature Metadata

label_name: trnV-GAC-rps12
feature_type: igs
region: IRa
length_bp: 1740
variable_sites: 5
pi: 0.0012
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rps12_p1	GATGACTTCCACCACGTCAA	CGTAAGGCAGAGTTGGGTTT	50.0	50.0	57.8	58.1	1805-1825 bp	1.0	78.9
trnV-GAC-rps12_p2	AACTGATGACTTCCACCACG	CGTAAGGCAGAGTTGGGTTT	50.0	50.0	57.8	58.1	1809-1829 bp	1.0	78.9
trnV-GAC-rps12_p3	ATGACTTCCACCACGTCAAG	CGTAAGGCAGAGTTGGGTTT	50.0	50.0	57.8	58.1	1804-1824 bp	1.0	78.9
trnV-GAC-rps12_p4	TGGTGGGGGAGTAAGTCAAA	CGTAAGGCAGAGTTGGGTTT	50.0	50.0	58.2	58.1	1870-1890 bp	1.0	78.8
trnV-GAC-rps12_p5	CTGATGACTTCCACCACGTC	CGTAAGGCAGAGTTGGGTTT	55.0	50.0	58.3	58.1	1807-1827 bp	1.0	78.0