Hotspot

petA-psbJ

LSC · 61653-62557 · 905 bp

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Pi 0.00727
MarkerSeek score 56.20
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
905
variable_sites
16
pi
0.00727
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTCGC ACGGTTCGTATTCTGGATTGG 50.0 47.6 57.9 58.1 1042-1055 bp 1.0 72.4
petA-psbJ_p2 GGCCTTTTGTTCTTCTTCGC GGTTCGTATTCTGGATTGGGT 50.0 47.6 57.9 57.7 1040-1053 bp 1.0 71.0
petA-psbJ_p3 GGCCTTTTGTTCTTCTTCGC CGGTTCGTATTCTGGATTGG 50.0 50.0 57.9 56.3 1041-1054 bp 1.0 67.7
petA-psbJ_p4 CGTCCAAGGCCTTTTGTTCT ACGGTTCGTATTCTGGATTGG 50.0 47.6 58.7 58.1 1049-1062 bp 1.0 67.4
petA-psbJ_p5 GGCCTTTTGTTCTTCTTCGC CGGTTCGTATTCTGGATTGGG 50.0 52.4 57.9 58.8 1041-1054 bp 1.0 66.9