Hotspot

ycf3-trnS-GGA

LSC · 45287-45894 · 608 bp

Back to Iseilema

Pi 0.00439
MarkerSeek score 52.60
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
608
variable_sites
8
pi
0.00439
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TTGGCTACAATCGAGGAGGT ACGGAAAGAGAGGGATTCGA 50.0 50.0 58.4 58.2 750-755 bp 1.0 76.7
ycf3-trnS-GGA_p2 TTGGCTACAATCGAGGAGGT TTCCAATGCTACGCCTTGAA 50.0 45.0 58.4 57.8 700-705 bp 1.0 76.5
ycf3-trnS-GGA_p3 GGCTACAATCGAGGAGGTCT ACGGAAAGAGAGGGATTCGA 55.0 50.0 58.6 58.2 748-753 bp 1.0 75.4
ycf3-trnS-GGA_p4 GGCTACAATCGAGGAGGTCT TTCCAATGCTACGCCTTGAA 55.0 45.0 58.6 57.8 698-703 bp 1.0 75.2
ycf3-trnS-GGA_p5 TTGGCTACAATCGAGGAGGT AGTTCCAATGCTACGCCTTG 50.0 50.0 58.4 58.5 702-707 bp 1.0 73.6