Hotspot

psaA-ycf3

LSC · 42660-43268 · 609 bp

Back to Iseilema

Pi 0.00262

MarkerSeek score 56.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 609
variable_sites: 3
pi: 0.00262
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GGGGCCCTTAGCTATTGTTC	TGGTTTGATCAAGCTGCTGA	55.0	45.0	58.0	57.7	831-840 bp	1.0	78.9
psaA-ycf3_p2	GTCTGGCCCATTCCTCAAAA	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.1	57.7	796-805 bp	1.0	78.5
psaA-ycf3_p3	GGTTCCGACGGACGAATAAT	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.8	57.7	734-743 bp	1.0	77.5
psaA-ycf3_p4	CTGGCCCATTCCTCAAAAGA	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.8	57.7	794-803 bp	1.0	77.3
psaA-ycf3_p5	TCTGGCCCATTCCTCAAAAG	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.8	57.7	795-804 bp	1.0	77.3