Hotspot

trnT-GGU-trnE-UUC

LSC · 15824-16292 · 469 bp

Back to Iseilema

Pi 0.00406

MarkerSeek score 43.10

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 469
variable_sites: 6
pi: 0.00406
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GACAGAAGTCATCGGTTCGA	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	57.4	58.3	546-555 bp	1.0	74.2
trnT-GGU-trnE-UUC_p2	GACAGAAGTCATCGGTTCGA	TGTCCTGAACCACTAGACGA	50.0	50.0	57.4	57.7	525-534 bp	1.0	74.1
trnT-GGU-trnE-UUC_p3	GACAGAAGTCATCGGTTCGA	CCTGAACCACTAGACGATGG	50.0	55.0	57.4	57.4	522-531 bp	1.0	71.6
trnT-GGU-trnE-UUC_p4	GACAGAAGTCATCGGTTCGA	CTGAACCACTAGACGATGGG	50.0	55.0	57.4	57.4	521-530 bp	1.0	71.6
trnT-GGU-trnE-UUC_p5	GACAGAAGTCATCGGTTCGAA	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	58.1	58.3	546-555 bp	1.0	70.7